DNA-In® HepG2 Transfection Reagent-细胞及转染-TwistDx,KAPA,Echelon,Monad莫纳,Jackson抗体,Biotechrabbit酶,Enzymatics,二代测序酶,ChromoTek,透析袋,默瑞生物

咨询热线:4006-400-850

收藏
当前位置:首页 > 默瑞产品 > 细胞及转染
细胞及转染 联系我们
< >
DNA-In® HepG2 Transfection Reagent

DNA-In® Cell-Specific Transfection Reagents are a line of transfection products developed by the lead members of the scientific team that invented the Lipofectamine® and Lipofectamine® 2000. These cel

  • 产品介绍
  • 规格参数
  • 售后服务

DNA-In® HepG2 

Formulated Specifically for HepG2 Cells 

DNA-In® Cell-Specific Transfection Reagents are a line of transfection products developed by the lead members of the scientific team that invented the Lipofectamine® and Lipofectamine® 2000. These cell-specific reagents offer maximum DNA delivery in targeted common cell lines.

DNA-In® HepG2 Transfection Reagent provides maximum transfection efficiency,  gene expression levels and cell viability in HepG2 cells, a human epithelial, hepatocellular carcinoma cell line,  commonly used as a model to study of human liver diseases, including investigation of liver metabolism and toxicity.

Features

  • Fast, simple, easy-to-follow protocol

  • One-step process: simply add DNA-In® HepG2 Transfection Reagent to diluted DNA, incubate 10 minutes, and add to pre-plated cells

  • Minimal toxicity - no need to replace medium after transfection

DNA-In® HepG2 Transfection Reagent  Quick-Start Protocol

<img src="https://www.mti-globalstem.com/image/catalog/Transfection-MTI/DNAIn-Transfection-Protocol_Animated.gif" alt="DNA-In HepG2 transfection protocol>

Superior Transfection Efficiency and Maximum Cell Viability

During its development and validation process, DNA-In® HepG2 Transfection Reagent underwent extensive testing in HepG2 cells. In head-to-head comparison studies, DNA-In® HepG2 Reagent consistently outperformed Lipofectamine® 2000 and Lipofectamine® 3000 transfection reagents to produce significantly higher transfection efficiency and cell viability.

The following charts show side-by-side comparisons of DNA-In® HepG2, Lipofectamine® 2000 (LF2K) and Lipofectamine® 3000 (LFK) in HepG2 cells:

DNAIn-Transfection-Protocol_Animated.gif

DNA-In® HepG2 Transfection Reagent provides Maximum Transfection Efficiency in HepG2 cells

Above, HepG2 cells were plated at  70% - 80% confluency. They were subsequently transfected with the EF-1 alpha promoter-GFP 24 hours after plating. Significantly higher efficiency  was consistently observed in HepG2 cells transfected with DNA-In® HepG2 Reagent (left image)  vs LF3K (right image) transfected cells.


版权所有:默瑞(上海)生物科技有限公司   

技术支持:易动力网络