EditPro™ Transfection Reagent has been optimized to deliver Cas9 mRNA or RNP with the associated guide RNA, into a wide range of adherent cell types. In addition, ssDNA constructs or single-stranded oligodeoxynucleotides (ssODN) can be mixed with the Cas9mRNA/gRNA or Cas9 protein/gRNA to promote homology-directed repair (HDR) to introduce targeted substitutions. It is well suited for co-transfection delivery of gene editing tools, therefore, into many primary cells and cell lines.
High Efficiency Cas9 mRNA Delivery
Below we demonstrate the efficiency of transfection of Cas9-encoding modified mRNA followed by detection with anti-Cas9 antibodies demonstrating high efficiency transfection and expression of the Cas9.
Figure 1. Antibody labeling of A549 cells and adult human dermal fibroblasts 24 hours post-transfection confirmed successful transfection of Cas9 mRNA using the EditPro™ Transfection Reagent.
Cas9-mediated INDEL Formation
The following data in HeLa cells demonstrates successful gene editing with EditPro™ via co-transfection of either Cas9 mRNA or Cas9 protein with the associated guide RNAs as indicated by the formation of INDEL bands.
Figure 2. Indel analysis following transfection with EditPro™ Transfection Reagent. HeLa cells were transfected using 1 or 2 µL of EditPro™ Transfection Reagent as indicated with Cas9 mRNA (modified)/ gBlock® U6sgRNA GAPDH oligo/ GFP mRNA (modified) or Cas9 protein/ gBlock® U6sgRNA GAPDH oligo/ GFP mRNA (modified). Genome-modification was analyzed using the T7Endo 1 assay.
High Efficiency – delivering Cas9 RNA and/or Cas9 protein for gene editing and DNA for homology-directed repair.
Easy to use – no special equipment required
Cost Effective – effective at low amounts of reagent resulting in low cost/well