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DNA-In® CRISPR 转染试剂

DNA-In® CRISPR Transfection Reagent Product Code: GST-2160 Availability: In Stock

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NEW DNA-In® CRISPR Transfection Reagent  

Specifically formulated for CRISPR/Cas9 transfection reagent

DNA-In® CRISPR transfection reagent is optimized for large plasmid delivery of CRISPR/Cas9 vectors  into a range of cell types. This reagent is especially well-suited for hard-to-transfect primary cells.  Below, a Cas9-GFP plasmid was delivered into primary fibroblasts, muscles cells, keratinocytes and others primary cells and commonly used cell types with high efficiency and low toxicity.

Highly efficient Cas9 delivery in primary cells

dna-in crispr transfection of fibroblastslipofectamine 2000 transfection of fibroblastslipofectamine 3000 transfection of fibroblasts

 Human dermal primary fibroblasts (above) were plated  to give ~70% plating density on the day of transfection. Cells were transfected with Cas9-GFP 24 hours after plating.  Cells were labelled with anti-GFP (green) to observe low expression of Cas9-GFP vector and Hoechst 3342 (blue) to view nuclei. DNA-In® CRISPR transfected cells (left image) show significantly higher Cas9  transfection efficiency vs cells transfected with competitor reagents (L2K, L3K). 

dna-in crispr transfection of keratinocyteslipofectamine 2000 transfection of keratinocyteslipofectamine 3000 transfection of keratinocytes

 Human primary keratinocytes (above) were plated  to give a 50-70% plating density on the day of transfection. Cells were transfected with Cas9-GFP 24 hours after plating.  Cells were labelled with anti-GFP (green) to observe low expression of Cas9-GFP vector and Hoechst 3342 (blue) to view nuclei. DNA-In® CRISPR transfected cells (left image) show significantly higher Cas9  transfection efficiency vs cells transfected with competitor reagents (L2K , L3K). 

dna-in crispr transfection of c2c12lipofectamine 2000 transfection of c2c12lipofectamine 3000 transfection of c2c12

 C2C12 mouse myoblasts  (above) were plated  to give a 50-70% plating density on the day of transfection. Cells were transfected with Cas9-GFP 24 hours after plating.  Cells were labelled with anti-GFP (green) to observe low expression of Cas9-GFP vector and Hoechst 3342 (blue) to view nuclei. DNA-In® CRISPR transfected cells (left image) show significantly higher Cas9  transfection efficiency vs cells transfected with competitor reagents (L2K, L3K). 

dna-in crispr transfection of skmclipofectamine 2000 transfection of skmclipofectamine 3000 transfection of skmc

 Human skeletal muscle cells (above) were plated  to give a 50-70% plating density on the day of transfection. Cells were transfected with Cas9-GFP 24 hours after plating.  Cells were labelled with anti-GFP (green) to observe low expression of Cas9-GFP vector and Hoechst 3342 (blue) to view nuclei. DNA-In® CRISPR transfected cells (left image) show significantly higher Cas9  transfection efficiency vs cells transfected with competitor reagents (L2K, L3K). 

Features

  • Optimized for large plasmid DNA containing Cas9/GFP/ guideRNA ( all-in-one).

  • High efficiency delivery of Cas9 expression vectors in hard-to-transfect primary cells.

Technical Data



DNA-In® is registered trademarks of Molecular Transfer, Inc.

Lipofectamine®, Opti-MEM® are registered trademarks of Thermo Fisher Scientific Inc.


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