HIP™ (Human Induced Pluripotent) Neurons are a mixed population of early differentiating neuronal progenitor cells derived from the BC1 iPSC line generated from adult CD34-positive bone marrow samples ( Chou et al, 2011). HIP™ Neurons offer a short culture time to mature neurons with reduced risk of over-proliferation. Cells are provided as frozen neuronal progenitor cells (NPCs) that can be differentiated to consistently produce high yields of functional, mature neurons and includes one bottle of our NeuralQ™ Basal Medium (GSM-9420) and GS21™ Neural Supplement (GSM-3100), and GS Pre-Coat Matrix Solution (GSM-9450).
1Chou, B. K., Mali, P., Huang, X., Ye, Z., Dowey, S. N., Resar, L. M., ... & Cheng, L. (2011). Efficient human iPS cell derivation by a non-integrating plasmid from blood cells with unique epigenetic and gene expression signatures. Cell research, 21(3), 518-529.
Figure 1 - HIP™ Neurons differentiated to neuronal subtypes - After five weeks of differentiation, cultures show (A) an abundance of MAP2-positive neurons express the synaptic markerSynapsin, (B) Astrocytes marked by GFAP, (C) ChAT-positiveCholinergic neurons and (D) Vglut1-positive Astrocytes andGlutamatergic neurons. Nuclei are labeled with the Hoechst3342(blue). Scale bars= 200 microns.
Used as in vitro Models of Neural Disease
HIP™ Neurons produce consistent, mature neuronal cultures for in vitro models of neural diseases. Through collaboration with SynAging SA [Nancy, France], HIP™ Neurons (human induced pluripotent neurons ) have been used to create reliable in vitroassays to study the neurodegenerative properties of amyloid betaoligomers (AbO) and alpha-synuclein oligomers (aSO) that mimic Alzheimer’s and Parkinson’s Diseases, respectively.
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Alzheimer’s Disease Model