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Biontex品牌METAFECTENE® PRO是针对哺乳动物细胞的新一代转染试剂
作者:moreybio 发布时间:2020-12-03 点击次数:3023
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METAFECTENE® PRO

METAFECTENE® PRO是针对哺乳动物细胞的新一代转染试剂。采用毒性优化专利技术(TOP技术),Biontex成功地降低了转染试剂对细胞的毒性,并显著提高DNA进入细胞的效率。除了能加强细胞的转染效率之外,METAFECTENE® PRO的另一个主要特点是提高细胞内基因的表达。

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TOP技术(Toxicity Optimization Module)

注:TOP技术(Toxicity Optimization Module)

毒性最小化是现代转染试剂达到最高效率和广泛应用性的首要前提条件。TOP技术的基本准则是模拟物质天然结构,加强生物降解能力,提高细胞耐受性。另外,通过物理化学修饰,降低脂质复合体的稳定性,从而促进DNA在细胞质的释放。

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Biontex产品

产品名称

货号

规格

METAFECTENE® PRO转染试剂

T040-1.0


1 ml 

METAFECTENE® PRO转染试剂

T040-2.0

2 X 1 ml

METAFECTENE® PRO转染试剂

T040-5.0

5 X 1 ml

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HIGHLIGHTS:

Suitable for DNA, mRNA, siRNA and miRNA transfection as well as cotransfection of DNA and RNA

Suitable for stable and transient transfection

Suitable for adherend and suspension cells

Minimized toxic effects

Optimized transfection rates

Free from serum inhibition

Comprehensive library of user reports

Wide range of references in specialist literature

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METAFECTENE® PRO is a liposome based transfection reagent consisting of a mixture of polycationic and neutral lipids. It is an advanced product based on METAFECTENE®. Structural changes of the comprising cationic lipids results in higher efficiency and lower toxicity.

METAFECTENE® PRO is part of a temporal development series of transfection reagents from Biontex, which includes in particular the following reagents: METAFECTENE® < METAFECTENE® PRO < K2® Transfection System < K4® Transfection System. With the development degree the probability of a successful transfection increases (see also General Selection Aid).

Low cytotoxicity and lack of serum inhibition allow for a wide range of applications. METAFECTENE® PRO is suitable for transient and stable transfection of mammalian cell lines and primary cells with DNA (plasmids, bacmids), RNA (mRNA, miRNA and siRNA) and modified nucleic acids (antisense oligonucleotides). Areas of application are particularly the production of proteins, antibodies, and viruses (e.g. adenoviruses, AAV, lentiviruses), cotransfections of different nucleic acids, genome editing (e.g. CRISPR/Cas9, CRISPR/Cpf1) and gene silencing (gene knockdown).

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